Figure 6.

Enhanced susceptibility of CaMBL1-silenced pepper plants to Xcv infection with the virulent (compatible) strain Ds1 and the avirulent (incompatible) strain Bv5-4a. A, Disease symptoms developed on the empty vector control (TRV:00) and silenced (TRV:CaMBL1) leaves infected by Xcv strains. Infected leaves were stained with DAB and trypan blue. Bars = 0.2 mm. B, Bacterial growth in leaves of the empty vector control (TRV:00) and CaMBL1-silenced (TRV:CaMBL1) plants 0 and 3 d after inoculation with Xcv strains (5 × 10⁴ cfu mL⁻¹). C, Quantification of H₂O₂ in leaves of empty vector control plants and CaMBL1-silenced plants after inoculation with Xcv strains using the xylenol orange assay. D, Measurement of electrolyte leakage from the leaf tissues at different time points after inoculation with Xcv strains. All experiments were repeated three times with similar results. Values are presented as means ± sd. Asterisks indicate significant differences between the means as determined by Student’s t test (P < 0.05).