Figure 9.

Disease development on the leaves of wild-type (WT), CaMBL1-OX, and Arabidopsis ortholog mutant (mbl1-1 and mbl1-2) plants inoculated with A. brassicicola. Leaves of 4-week-old Arabidopsis plants were inoculated with 10-μL droplets of 5 × 10⁴ spores mL^–1. A, Schematic representation of T-DNA insertion sites in mbl1-1 (SALK_038821) and mbl1-2 (SALK_121641) plants and the genomic structure of the At1g78830 gene. The At1g78830 exon is represented by the black box. B, RT-PCR analysis of expression of the mbl1-1 and mbl1-2 genes 15 h after inoculation with Pst DC3000 avrRpm1. C, Disease symptoms on leaves 5 d after inoculation. D, Microscopic images of fungal structures and damaged cells of infected leaves stained with trypan blue. Bars = 0.1 mm. E, Quantification of disease development based on the average diameter of lesions caused by A. brassicicola. The size of lesions is an average of 30 lesions per line. Values are presented as means ± sd. Different letters indicate significant differences from three independent experiments based on the lsd test (P < 0.05). [See online article for color version of this figure.]