Figure 4.

Identification of DNA-binding sequences of StMYB1R-1. A, Summary of putative StMYB1R-1 DNA-binding sequences. Q9 protein-binding microarray analysis was performed using a StMYB1R-1-DsRed fusion protein. A total of 1,028 probe sequences that produced high signal intensities were selected for further analysis. B, Signal intensities of the GGATAA sequence and single nucleotide substitution derivatives using the Q9 protein-binding microarray. W, Wild-type sequence; 1 to 9, single nucleotide substitution variants (number corresponds to the position of the nucleotide substitution). One of the probe sets that is irrelevant with the GGAAA sequences was used as negative control (N). [See online article for color version of this figure.]