Figure 8.
Up-regulation of drought stress-responsive genes in transgenic potato plants overexpressing StMYB1R-1. A, Transcript levels of putative stress-responsive genes selected based on cDNA microarray analysis. Three-week-old wild-type potatoes were incubated on 3MM paper for 1 or 6 h to simulate drought stress, and then total RNA was extracted. Transcript levels for each gene were analyzed using gene-specific primers and semiquantitative RT-PCR. B, Transcript levels of drought stress-responsive genes are enhanced in transgenic potato plants overexpressing StMYB1R-1. Potato β-tubulin was used as a quantitative control (Nicot et al., 2005).