Table I. Xyloglucan content and molecular mass in transgenic PtxtXET16-34-OE lines and wild-type plants.

Xyloglucan was extracted from primary and secondary wall-developing xylem (xylem 1 and xylem 2, respectively) with both 4% KOH and 24% KOH. Sequential extractions were performed using tissues pooled from five to six individuals for each line with two (xylem 1) or three (xylem 2) technical replicates. The average xyloglucan content of each transgenic line was significantly different from that of the wild type based on ANOVA followed by t tests (P ≤ 0.05), as indicated by asterisks. Weight-averaged molecular mass [M_(w)] was calculated for the main peak illustrated in Figure 5. ND, Not detected.

Line	Xylem 1				Xylem 2
		4% KOH	24% KOH	24% KOH M(w)	24% KOH in Total Xyloglucan	4% KOH	24% KOH
	mg g−1 dry wt		kD	%	mg g−1 dry wt
2-1	1.13*	12.63*	126	91.8	ND	0.95*
2-2	1.74*	18.43*	110	91.4	ND	1.09*
5-2	1.50*	8.58*	152	85.1	ND	0.56*
Wild type	2.91	6.18	148	68.0	ND	1.40