Figure 5. Apoptosis is induced in the majority of Ba/F3 cells with only eight molecules of the 23 kDa protein inhibitor of BCR-ABL.
Images of a (A)healthy and (B)dying cell observed using oblique illumination. Scale bar is 5 µm. (C) Histogram of cell death in Ba/F3 cells. From left to right, cells loaded with 100nm lipid nanocapsules containing AGT-PP1-1 for six hours and left unphotolyzed; cells in which 4 empty lipid nanocapsules were photolyzed; cells in which 1–2 lipid nanocapsules containing AGT-PP1-1 were photolyzed; cells in which 3–4 lipid nanocapsules containing AGT-PP1-1 were photolyzed; and cells in which 4 lipid nanocapsules containing AGT-PP1-1 were photolyzed in the presence of 1 ng/ml IL-3. For each bar in the histogram, N=25 cells.
