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. 2003 Dec 10;100(26):15434–15439. doi: 10.1073/pnas.2535024100

Fig. 6.

Fig. 6.

Structural determinants of SB 203580 sensitivity. (A) Effect of SB 203580 on cellular RICK kinase activity. COS-7 cells were transiently transfected with pPM7-RICK-KRdCst expression plasmid (1.3 μg per well) plus either control vector or pPM7 plasmids encoding RICK or RICK-T95M (0.2 μg per well). After metabolic labeling with [32P]orthophosphate and precipitation using StrepTactin beads, radioactively labeled RICK-KRdC was detected by autoradiography (blots 1, 3, and 5) before immunodetection with StrepTactin-horseradish peroxidase (blots 2, 4, and 6). (B) Amino acid residues surrounding Thr-106 in p38 were aligned with the corresponding sequences of various protein kinases binding to the PI 51 matrix. With the exception of CK1δ, all kinases possessing a larger amino acid residue at the indicated position (gray background) show significantly higher IC50 values than those having a threonine residue at this site.