Fig. 1.

Simvastatin increases secreted ET-1 protein levels in SH-SY5Y cells. Cells were incubated with 100 nM simvastatin or vehicle (10 μM ethanol) for 72 and 144 h. Conditioned media were collected and ET-1 protein levels were determined using the Endothelin-1 TiterZyme® Enzyme Immunometric Assay (Assay Designs) (see Materials and Methods). Results are the means ± SEM of three independent experiments performed in triplicate. ^***p≤0.001 as compared with control