Fig. 3.

Simvastatin increases NFATc3 protein levels in the nuclear fraction of SH-SY5Y cells. Cells were treated with simvastatin (100 nM) for different periods after which time the nuclear fraction was isolated using the Nuclear Extract Kit (Active Motif) (see Materials and Methods). NFATc3 protein levels were determined in the nuclear fraction by Western blot analysis as shown by a representative blot. Band density was quantified by densitometry and expressed relative to lamin A using the Stratagene Eagle Eye II system. Data are percentage change of control and are the means ± SEM of three independent experiments. *p≤0.05 and **p≤0.01 as compared with control cells