Table 2. Changes in gene expression profile of angiogenesis related pathways determined by RT-PCR array after 3 days of Sham, BCNR and SIL treatment.
Pooled penile Total RNA from 3 days Sham BCNR and BCNR+SIL were subjected to RT PCR array utilizing the Rat Angiogenesis RT2 Profiler™ PCR Array. List of genes up or down regulated) by BCNR n comparison with Sham and BCNR+SiL in comparison with BCNR. The ratios between the Sham vs. BCNR and BCNR vs. BCNR+SIL were normalized by RPLP1, RPLP3 and LDH housekeeping genes and were calculated for the assays performed in quadruplicates. Genes that were +2.0 fold up regulated are labeled in red, genes down regulated by −2.0 are labeled in blue. Genes regulated by less than ± 2 fold are labeled in black. It is considered significant those genes that shows ±2 fold changes
| Sham vs BCNR | BCNR vs. SIL | ||
|---|---|---|---|
| Symbol | Gene name | Fold change | Fold change |
| Akt1 | Thymoma viral proto-oncogene 1 | −2.65 | 1.73 |
| Angpt1 | Angiopoietin 1 | 2.14 | −2.48 |
| Anpep | Alanyl (membrane) aminopeptidase | −2.65 | 2.31 |
| Ctgf | Connective tissue growth factor | 3.35 | −2.45 |
| Ecgf | Endothelial cell growth factor 1 (platelet-derived) | −2.27 | 1.81 |
| Efna2 | Ephrin A2 | −3.15 | 3.32 |
| Efna5 | Ephrin A5 | −2.01 | 1.33 |
| Egf | Epidermal growth factor | 1.51 | 2.16 |
| Ereg | Epiregulin | −4.77 | 2.01 |
| Fn1 | Fibronectin 1 | −2.04 | −1.38 |
| Itga5 | Integrin alpha 5 | −2.01 | 1.42 |
| Kdr | Kinase insert domain protein receptor | 1.22 | 2.12 |
| Mmp3 | Matrix metallopeptidase 3 | −2.43 | 1.42 |
| Mmp9 | Matrix metallopeptidase 9 | −7.49 | 3.51 |
| Pdgfa | Platelet derived growth factor, alpha | −2.43 | 3.63 |
| Serpinb5 | Serine (or cysteine) peptidase inhibitor, | 2.77 | −2.61 |
| TGFb2 | Transforming growth factor, beta 2 | 6.04 | −2.89 |
| Thbs4 | Thrombospondin 4 | −4.61 | 1.02 |
| Timp2 | Tissue inhibitor of metalloproteinase 2 | −2.35 | 1.14 |