Table 3. Changes in Gene expression profile of angiogenesis related pathways determined by RT-PCR array after 45 days BCNR and SIL treatment.
Pooled penile Total RNA from 45 days Sham, BCNR and BCNR+SIL were subjected to RT PCR array utilizing the Rat Angiogenesis RT2 Profiler™ PCR Array.. The ratios between the sham vs. BCNR and BCNR vs. BCNR+SIL were normalized by RPLP1, RPLP3 and LDH housekeeping genes and were calculated for the assays performed in quadruplicates Genes that were +2.0 fold up regulated are labeled in red, genes down regulated by −2.0 are labeled in blue. Genes regulated by less than ± 2 fold are labeled in black.
| Sham vs BCNR | BCNR vs. SIL | ||
|---|---|---|---|
| Symbol | Gene name | Fold change | Fold change |
| Ctgf | Connective tissue growth factor | 2.99 | −3.00 |
| Cxcl2 | Chemokine (C-X-C motif) ligand 2 | 2.01 | −2.01 |
| Efna1 | Ephrin A1 | 1.22 | 2.09 |
| Efna2 | Ephrin A2 | 1.26 | 2.06 |
| Epas1 | Hypoxia fibroblast factor | 2.00 | −2.00 |
| Ereg | Epiregulin | −14.83 | 6.57 |
| Fn1 | Fibronectin 1 | −4.33 | −2.44 |
| Id1 | Inhibitor of DNA binding 1 | −2.00 | 1.25 |
| Ifnb1 | Interferon beta 1, fibroblast | 2.00 | 4.26 |
| Il1b | Interleukin 1 beta | 2.01 | −1.16 |
| Itga5 | Integrin alpha 5 | −2.13 | 2.43 |
| Mapk14 | Mitogen activated protein kinase 14 | 3.00 | −1.10 |
| Mmp9 | Matrix metallopeptidase 9 | −1.50 | 8.21 |
| Npr1 | Natriuretic peptide receptor 1 | −3.40 | 4.08 |
| Nrp1 | Neuropilin 1 | −1.45 | 3.04 |
| Nrp2 | Neuropilin 2 | −2.49 | 5.12 |
| Pdgfa | Platelet derived growth factor, alpha | −2.00 | 2.00 |
| Pdgfb | Platelet derived growth factor, B polypeptide | −3.28 | 2.00 |
| Pecam | Platelet/endothelial cell adhesion molecule | −2.62 | −1.64 |
| Thbs4 | Thrombospondin 4 | −4.18 | −1.27 |
| Timp2 | Tissue inhibitor of metalloproteinase 2 | −2.13 | 1.17 |
| Tnf | Tumor necrosis factor | 2.20 | 2.4 |
| Vegfa | Vascular endothelial growth factor A | −1.20 | 2.76 |
| Vegfb | Vascular endothelial growth factor B | −1.36 | 2.67 |