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. 2003 Dec 5;100(26):15458–15462. doi: 10.1073/pnas.2136651100

Fig. 1.

Fig. 1.

Isolated vacuoles from yeast display ATP-dependent and vacuolar H+-ATPase-dependent transport of arginine into the vacuole. (A) Uptake of [14C]arginine into isolated vacuoles from wild-type yeast (B-11718) in the presence (•) and absence (○) of ATP. Results shown are typical of those obtained from six independently derived uptake experiments. (B) Lineweaver–Burk plot with 95% confidence limits, showing first-order kinetic uptake, Km = 0.02 mM, of [14C]arginine into isolated vacuoles from wild-type yeast, B-11718. (C) Rate of uptake of [14C]arginine into isolated vacuoles from wild-type (WT) yeast, B-11718; yeast lacking the CUP5-encoded V0 subunit of the vacuolar H+-ATPase (cup5-Δ), B-12074; yeast lacking the VMA13-encoded V1 subunit of the vacuolar H+-ATPase (vma13-Δ), B-13046; and yeast lacking Btn1p (btn1-Δ), B-13048. Results were obtained from four independently derived preparations of vacuoles from each strain. All isolated vacuoles shown were prepared from strains grown on YPD.