Influence of light dose on phototoxicity of free ZnPc and ZnPc-loaded nanoparticles. The cells were incubated for 4 h, at an equivalent drug dose of 5-pg ZnPc (250 μL), washed, and irradiated with red light (660 nm). MTT assay was performed 24 h after light exposure. Each data point represents the mean (±SD) of n = 10 determinations.
Notes: *Significant difference (P < 0.05), **No significant difference (P > 0.05).
Abbreviations: ZnPc, zinc phthalocyanine; MTT, 3-(4,5-dimethyl-thiazol-2-yl)-2,5-biphenyl tetrazolium bromide; SD, standard deviation.