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. Author manuscript; available in PMC: 2011 Apr 13.
Published in final edited form as: Dev Dyn. 2008 May;237(5):1389–1398. doi: 10.1002/dvdy.21545

Figure 2. Genome-wide Increases in Histone H3 Tail Acetylation and Tri-Methylation During Embryonic Stem Cells Differentiation.

Figure 2

Graphs depict mean western blot band density normalized to both pan-histone H4 band intensity and each time-course's maximum observed density ± standard error. * = p < 0.05 by Student's t-test. Typical blot image are provided below each graph. (A): Acetyl lysine 9 on histone H3 is nearly undetectable in undifferentiated human embryonic stem cells and increases steadily from differentiation day 4 to 17. (B): Acetylated lysine 9 on histone H3 increases significantly during the first four days of mouse embryonic stem cell differentiation and remains consistently elevated thereafter. (C): Tri-methylation of lysine 9 on histone H3 is low in undifferentiated human embryonic stem cells and increases significantly during differentiation. (D): Tri-methylation of lysine 9 on histone H3 is low in mouse embryonic stem cells differentiation and shows an oscillatory but generally increasing level during differentiation.

Abbreviations: AK9 = Acetyl Lysine 9 on Histone H3. TMK9 = Tri-Methyl Lysine 9 on Histone H3. H4 = pan H4 normalization control. ESC = Embryonic stem cell.