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. Author manuscript; available in PMC: 2011 Apr 13.
Published in final edited form as: Dev Dyn. 2008 May;237(5):1389–1398. doi: 10.1002/dvdy.21545

Figure 4. RNA Abundance of Key Developmentally Regulated Loci During Cardiac Directed Differentiation of H7 Human Embryonic Stem Cells.

Figure 4

Human embryonic stem cells were plated into a high-density monolayer, switched to RPMI supplemented with B27 and sequentially exposed to Activin A and BMP4. RNA was collected from the cells in colony propagation (undifferentiated), on the day of induction (day 0), day 2, day 5, day 7, day 10 and day 14. SYBR Green quantitative reverse transcription PCR was performed with primers amplifying Oct4, Sox1 (ectoderm marker) or Brachyury T (mesoderm marker). Values are reported as fold over undifferentiated, with the left vertical-axis for Sox1 and Brachyury and the right vertical-axis for Oct4. Oct4 (open circles and a dashed line) rapidly declined with induction of differentiation, becoming nearly undetectable by day 5. Sox1 shows no significant expression at any time point observed. Brachyury T shows a spike of expression around two days after induction, followed by a rapid decline to nearly undetectable levels by seven days after induction.