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. 2011 Feb 22;152(5):1961–1969. doi: 10.1210/en.2010-1065

Fig. 2.

Fig. 2.

Insm2 in developmental and adult tissues. A, Insm2 mRNA is shown in mouse embryonic development from E6.5 to E18.5. B, The Insm2 mRNA level in the mouse brain is gradually decreased after 4 wk postnatally. mRNAs are equally loaded as indicated by 18S and 28S. C, INSM2 transcript is shown in human adult tissues. D, Insm2 transcript is shown in adult mouse tissues. E, Western blots with INSM2 antibody detect a single band at 60 kDa in mouse pancreas and brain tissues as well as in mouse insulinoma MIN6 and rat pheochromocytoma PC12 cell lines. INSM2 is strongly expressed in mouse Neuro2a (N2a) cells, but not in the HeLa cells. GAPDH served as a loading control across all the lanes.