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Mean Kiss1 mRNA copy number, as determined via qPCR, in the MeA and AVPV of male and female mice that were GDX and treated with E2 or nothing. In both regions, there were significantly more Kiss1 mRNA-expressing cells in E2-treated mice compared with untreated GDX mice. *, Significantly different (P < 0.05) from GDX mice in same region. Cast, Castrated; OVX, ovariectomized.
