Table 1. Quantification of glycoprotein interactions by BiFC assay.
Quantification of gB – gH/gL interactions. For each of the glycoprotein combinations, the number of cells expressing gB, gH/gL, and YFP were counted from two separate experiments. A ratio of the number of YFP cells to the number of total cells expressing gB and gH/gL was calculated and presented as a percentage.
| gB construct | gH/gL |
# YFP cells # gB and gH/gL stained |
|---|---|---|
| EBV gBN | EBV gHC EBV gL | 83% |
| EBV/Rh (456-628) gBN | EBV gHC EBV gL | 93% |
| EBV/Rh (456-807) gBN | EBV gHC EBV gL | 84% |
| EBV/Rh (628-807) gBN | EBV gHC EBV gL | 83% |
| EBV/Rh (1-807) gBN | EBV gHC EBV gL | 93% |
| EBV gBN | Rh-LCV gHC Rh-LCV gL | 68% |
| EBV/Rh (456-628) gBN | Rh-LCV gHC Rh-LCV gL | 72% |
| EBV/Rh (456-807) gBN | Rh-LCV gHC Rh-LCV gL | 79% |
| EBV/Rh (628-807) gBN | Rh-LCV gHC Rh-LCV gL | 59% |
| EBV/Rh (1-807) gBN | Rh-LCV gHC Rh-LCV gL | 91% |