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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2010 Nov 4;31(2):392–398. doi: 10.1161/ATVBAHA.110.213181

Figure 2.

Figure 2

Phosphorylation-dependent association of eNOS and Pin1 in BAECs. A, BAEC lysates were immunoprecipitated (IP) with anti-Pin1 antibody and immunoblotted (IB) with anti-eNOS antibody. B, BAEC lysates were immunoprecipitated (IP) with anti-eNOS antibody and immunoblotted (IB) with anti-Pin1 antibody. C, BAECs were either not pretreated or pretreated with PD98059 (50 μmol/L for 1 h) prior to immunoprecipitation (IP) with anti-eNOS antibody and immunoblotting (IB) with either anti-Pin1 or anti-eNOS antibodies. Results are representative of at least 3 different experiments.