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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2010 Nov 4;31(2):392–398. doi: 10.1161/ATVBAHA.110.213181

Figure 3.

Figure 3

Phosphorylation-dependent association of eNOS and Pin1 following ectopic expression of Pin1 with either wild-type (WT) eNOS or phospho-mimetic (S116D) eNOS. COS-7 cells were transfected with either WT eNOS or S116D with and without cotransfection with V5-tagged Pin1. A, cell lysates were immunoprecipitated (IP) with anti-V5 antibody and immunoblotted (IB) with anti-eNOS antibody. B, cell lysates were immunoprecipitated (IP) with anti-V5 antibody and immunoblotted (IB) with anti-V5 antibody. Similar results were obtained in 3 experiments.