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Representative current traces from single homoheptameric mutant αHL pores showing blockades by βCD. All traces were recorded under symmetrical buffer conditions with 1 M NaCl and 10 mM Tris·HCl, pH 7.5. The βCD (40 μM) was added to the trans or the cis chamber as indicated. (Left) Traces recorded at –40 mV. (Right) Traces recorded at +40 mV. (A)PNN-E with βCD in the trans or the cis chamber. (B) PNN-D. (C) PNN-R. (D) PNN-K.
