Table 1.
% Tetramer+ cells*: |
|||||||
---|---|---|---|---|---|---|---|
Macaque | Blood | Vagina | Cervix | Lymph node | Spleen | Jejunum | Colon |
97D248 | 0.4 | 6.4 | 13.2 | 2.7 | NT | NT | NT |
297.98 | 0.1 | 1.6 | 3.0 | 0.6 | NT | NT | NT |
226.88 | 0.1 | 0.3 | NT | 0.5 | 0.6 | 0.5*** | 0.2** |
214.87 | 0.5 | 3.4 | 2.0 | 4.9 | 5.6 | 6.7** | 5.1** |
252.90 | 0.1 | 1.8 | 2.1 | 1.5 | NT | 1.8 | 2.0 |
541.99 | 4.5 | 13 | 16 | 5.3 | 9 | 14 | 12 |
430.93 | 0.6 | 4.0 | 2.2 | 1.7 | NT | 2.3*** | 2.9*** |
Percentage of Mamu A*01/SIV Gag181–189 tetramer-binding cells after gating on CD3+CD8+ cells.
Lamina propria lymphocytes (LPL) isolated from jejunum and colon with type II collagenase after removal of intraepithelial lymphocytes (IEL) using EDTA.
Mixed population of IEL and LPL isolated from the jejunum using type II collagenase alone.