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. Author manuscript; available in PMC: 2011 Dec 10.
Published in final edited form as: Circ Res. 2010 Oct 21;107(12):1454–1459. doi: 10.1161/CIRCRESAHA.110.228361

Figure 3. Mutation in the PDZ-binding motif of hSlo-β1 prevented hSlo-β1 ubiquitination and degradation.

Figure 3

Sequence alignment of human, rabbit, cattle, rat, and mouse KCNMB1 cDNA shows a conserved PDZ-binding motif (A). ▼: applied mutation site. 72-h after co-transfection with atrogin-1 and ubiquitin, Flag-hSlo-β1V146A expression was significantly higher than that of Flag-hSlo-β1 wt (B). Immunoprecipitates of anti-Flag antibody against HEK293 cell lysates with following transfection conditions: no transfection, Flag-hSlo-β1/ubiquitin, Flag-hSlo-β1/atrogin-1/ubiquitin, Flag-hSlo-β1V146A/atrogin-1/ubiquitin and Flag-hSlo-β1/atrogin-1ΔF/ubiquitin, were resolved and blotted against anti-ubiquitin antibody (C).