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. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: Cancer Res. 2010 Dec 2;71(2):371–382. doi: 10.1158/0008-5472.CAN-10-3221

Figure 1. AKT1 interacts with menin.

Figure 1

(A) HEK-293 cells were cotransfected with FM and HA-AKT1 followed by co-IP (left panel). Whole cell lysates from HEK-293 cells transfected with HA-AKT1 were used for FLAG pull down assay (right panel). (B) The pancreas of three-month-old mice was used for co-IP. (C) Whole cell lysates from HEK-293 cells infected with various adenovirus (10 MOI) (multiplicity of infection) were used for co-IP. (D) Purified GST-AKT1 or GST-AKT1 kinase was used for FLAG pull down assay.