Skip to main content
. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: Cancer Res. 2010 Dec 2;71(2):371–382. doi: 10.1158/0008-5472.CAN-10-3221

Figure 2. Menin suppresses AKT kinase activity and AKT1 mediated effects.

Figure 2

(A) The cytoplasmic extracts from 3×105 KO and KOM cells were subjected to IB. The quantified results from four independent experiments are shown in the graphs. The protein levels were normalized against the respective total protein and α-tubulin levels. (B) An in vitro kinase assay was performed with endogenous active AKT (S473) immunoprecipitated from the cytoplasmic extracts of KO or KOM cells. The quantified results from three independent experiments are shown on the bottom. (C) Menin-null MEFs were infected with various adenovirus (100 MOI) followed by cell proliferation assay. Cell lysates from the cells were subjected to IB. (D) 1×104 menin-null MEFs were seeded in 6-well plates and infected with various adenovirus (100 MOI) followed with cisplatin and insulin treatment and processed to detect release of free nucleosomal DNA.