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. 2010 Dec 8;30(49):16651–16661. doi: 10.1523/JNEUROSCI.3211-10.2010

Figure 8.

Figure 8.

Effects of BKIP-1 on SLO-1 channel functional properties in Xenopus oocytes. A, Representative macroscopic current traces in inside-out patches from oocytes expressing SLO-1 alone (Control) or SLO-1 plus BKIP-1 (+BKIP-1) in response to voltage steps (−80 to +160 mV at 20 mV intervals and 50, 100, or 300 ms pulse duration) at different [Ca2+] in the bath solution. B, The G–V relationships of Control and +BKIP-1 at different [Ca2+] were fitted to the Boltzmann function G = Gmax/[1 + exp(V50 − V)/k], where G is the conductance at voltage V, Gmax is the maximal conductance, V50 is the voltage at which G = 0.5 Gmax, and k is the slope factor. C, Effects of BKIP-1 on SLO-1 V50 at different [Ca2+]. BKIP-1 increased V50 at 10 μm [Ca2+] but decreased V50 at 100 μm and 1 mm [Ca2+]. The difference was statistically significant at all [Ca2+] (p < 0.01, unpaired t test). D, Effect of BKIP-1 on SLO-1 activation kinetics. Activation time constant was determined by a single-exponential fit to the current trace at each voltage step. BKIP-1 significantly slowed SLO-1 activation at 10 μm [Ca2+] but not at the higher [Ca2+], and this effect of BKIP-1 was independent of membrane potential (two-way ANOVA). Data are shown as mean ± SE. The numbers of patches (n) analyzed were 10 in all cases except for Control (n = 12) and +BKIP-1 (n = 13) at 1 mm [Ca2+].