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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: Anal Chem. 2010 Nov 29;82(24):10102–10109. doi: 10.1021/ac1020744

Figure 5.

Figure 5

Analysis of in vitro transcribed RNA standards processed on-chip using one-step RT-PCR. (A) Representative electropherograms shows that each RNA standard (100 copies each) can be accurately identified in the presence of all four primer pairs using the microdevice. A negative control containing no template verifies the absence of carryover between analyses. (B) Sensitivity analysis, as a function of initial RNA template concentrations, shows that the microdevice can detect hCoV-OC43, FLUBV, FLUAV, and hMPV RNA at 25, 50, 100, and 100 copies respectively with S/N > 5 (n=3).