Skip to main content
. 2003 Dec 5;100(26):15560–15565. doi: 10.1073/pnas.2536699100

Fig. 1.

Fig. 1.

CD63 and H,K-ATPase localization and association in parietal cells. (A) Rat stomach cryosections costained with anti-HKα pAb (green) and anti-CD63 mAb (red). (Scale bar is 50 μm.) (B) TVE samples from the 27% (6 μg) and 32% (14 μg) sucrose interfaces (kind gift from C. T. Okamoto) were immunoblotted with anti-HKβ mAb or anti-CD63 mAb (35). (C) Purified preparations of hog TVEs (13.3 μg) blotted with anti-HKβ mAb or biotinylated tomato lectin. (D) Purified TVEs incubated in 2% CHAPS (CH) or 1% Triton X-100 (Tr) lysis buffer. TVEs were incubated with streptavidin beads that were (+ lectin) or were not (–lectin) preconjugated with biotinylated tomato lectin. Precipitated material was immunoblotted with anti-CD63 mAb. The lane labeled TVE lysate contains 25 μg of lysate.