Skip to main content
. 2003 Dec 5;100(26):15560–15565. doi: 10.1073/pnas.2536699100

Fig. 2.

Fig. 2.

The HKβ coprecipitates with CD63. COS cells were transfected with the HKβ alone, CD63-FL alone, or the HKβ and CD63-FL (HKβ + CD63-FL). Cells were lysed in 2% CHAPS, 1% Brij 96 (Br), or 1% Triton X-100 (Tr) and immunoprecipitated with anti-FLAG pAb. The second lane was immunoprecipitated from a mixture of cell lysates from cells separately transfected with CD63-FL and the HKβ. Precipitated proteins were blotted with anti-HKβ mAb or anti-Lamp-1 mAb.