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. 2003 Dec 5;100(26):15560–15565. doi: 10.1073/pnas.2536699100

Fig. 4.

Fig. 4.

Colocalization to intracellular vesicles is specific for proteins that interact with CD63. (A) COS cells were transfected with AQP4 alone or were cotransfected with AQP4 and CD63-FL (AQP4 + CD63-FL). The cells were lysed in 1% Triton X-100 and immunoprecipitated with anti-FLAG mAb. The immunoprecipitated material and the cell lysates were probed with anti-AQP4 pAb. (B) COS cells were transfected with either AQP4 (green) or AQP4 and CD63-FL (red). AQP4 was detected with anti-AQP4 pAb, and CD63 was detected with anti-FLAG mAb. (Scale bar is 10 μm.)