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The HKβ is endocytosed from the cell surface into CD63-positive vesicles. COS cells were transfected with the HKβ (A–D) or with β-subunit and CD63-FL (E–H). Surface β-subunit is labeled with Cy5 (blue; A and E), internalized β-subunit is labeled with fluorescein isothiocyanate (green; B and F), and CD63-FL is labeled with rhodamine (red; C and G). (Scale bar is 10 μm.)
