Fig. 4.

Complex relationships between endocrine factors and gene transcripts in honey bees. (A–D) The correlative relationship between ilp1 and vg is positive (vg⁺aa^–, N=10; vg^–aa⁺, N=8; vg^–aa^–, N=11), with the exception of RNAi controls that received amino acid (aa⁺) supplementation (vg⁺aa⁺, N=13; B). Gene expression levels are given as log-transformed quantities relative to β-actin expression (logRQ). (E,F) The correlative relationship between ilp2 and JH is conditional on vg. (E) For vg⁺ controls (vg⁺aa⁺, N=7; vg⁺aa^–, N=8), ilp2 and JH are positively correlated. (F) After vg RNAi (vg^–aa⁺, N=13; vg^–aa^–, N=15), however, the relationship is inversed and ilp2 and JH are negatively correlated.