Fig. 4.

Transfected KIF17b colocalizes with TB-RBP and Y and H element containing RNA in rat hippocampal neurons. Plasmid constructs containing KIF17b and TB-RBP cDNAs cloned in frame in pEGFPc2 and pDsRed1 vectors, respectively, were transfected into cultured rat embryonic hippocampal neurons with Nupherin-neuron and FuGENE 6 reagent. (A) GFP–KIF17b fusion protein. (B) TB-RBP–DsRed fusion protein. (C) Merged image. Arrowheads indicate the colocalization of KIF17b and TB-RBP in the dendritic processes. (D–H) Transfected Y and H element RNA colocalizes with TB-RBP and KIF17b. Cells expressing GFP–TB-RBP or GFP–KIF17b were retransfected 36 h later with Alexa Fluor 546 RNA probe and show colocalization (arrows) of RNA with GFP–TB-RBP (G) and GFP–KIF17b (H). Control single transfections with TB-RBP–DsRed (D), GFP–KIF17b (E), and Alexa Fluor 546 (F) riboprobes. Dual-channel confocal imaging was performed within 30 min of transfection of the riboprobe.