Fig 2.

IGF-1 at the low dose attenuated LPS-induced hypomyelination and decreases in the number of mature OLs. The extent of myelination was estimated by MBP immunohistochemistry at both the bregma (A, B&C) and the dorsal hippocampal level (D, E&F). The mature OLs were identified using APC antibody (G, H&I). MBP expression was quantified using a grading method with scores from 0 to 3, where 0=no staining, 1= weak but detectable staining, 2=reduced staining compared to 3 but stronger than 1, and 3=normal. LPS treatment resulted in a significant decrease of MBP scores in the white matter (J). The number of APC+ mature OLs was also reduced in LPS treatment compared to that in the control (K). IGF-1 significantly attenuated LPS-induced reduction in MBP expression as well as decrease of mature OL number. Scale bar: A though F: 200 μm, G though I: 50 μm. *p<0.01 vs LPS treatment.