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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Nucl Med. 2010 Apr 15;51(Suppl 1):128S–136S. doi: 10.2967/jnumed.109.068239

FIGURE 4.

FIGURE 4

Imaging fate of transplanted ESC-derived cardiomyocytes and ESC-derived endothelial cells. (A) Human ESC-derived cardiomyocytes stably expressing Fluc-eGFP double-fusion reporter gene were injected into ischemic myocardium of immunodeficient SCID mice. Longitudinal bioluminescence imaging showed that signal activity fell drastically within first 3 wk of transplantation and remained stable thereafter, with no evidence of tumorigenesis. (Reprinted with permission of (29).) (B) Mouse ESC-derived endothelial cells stably expressing Fluc-eGFP double fusion reporter gene were injected into ischemic myocardium of syngeneic SV129 mice. Longitudinal bioluminescence imaging showed similar pattern of acute donor cell loss, with about 1% signal intensity (relative to day 2) at 8 wk. Control animals injected with phosphate-buffered saline showed no imaging signals, as expected. (Reprinted with permission of (31).) CM = cardiomyocyte; EC = endothelial cell.