Fig. 5.

The MIS type II receptor is expressed in normal rat cervical tissue. (A) Lysates prepared from rat ovary, testes, cervix, lower vagina, and small intestine were probed with Ab against the MIS type II receptor. The 63-kDa receptor is present in OVCAR8 cells at endogenous levels and overexpressed in OVCAR8 cells transfected with the MIS type II receptor construct. Analogous bands are present in rat ovarian, testicular, and cervical tissue but not in the tissue prepared from the non-Mullerian tissues from the lower vagina or small intestine. (B) The 63-kDa receptor band is not seen when the blot is probed with preimmune serum, confirming specificity of the Ab.