Figure 1.

Analyses of Amhr2-Cre-induced recombination in murine uteri. (Panel A) Amhr2-Cre mice were crossed with LacZ and Yfp reporter mice. Amhr2-Cre induced β-galactosidase activity and direct YFP fluorescence was detected in stroma (ES) but not in epithelial cells of uteri (arrowheads and asterisks) or in their respective controls (A, insets). (Panel B, a) PCR of genomic DNA collected from the epithelium and stroma of APC^cko tumors using laser capture microdissection and from whole uterus, ovary, oviduct, and tail was used to detect recombined 500 bp floxed allele. The unrecombined flox APC allele (430 bp) was present in all tissue examined. (Panel B, b) Western blot analyses of uterine lysates showing increased expression of β-catenin, TCF1, LEF1, and Cyclin d1 in APC^cko compared to control mice. β-actin was used a loading control. (Panel C) Immunolocalization of β-catenin in 4-wk-old (a, b) and 5-month old (c, d) uteri of control and APC^cko mice. Inset in b is a higher magnification image of area outlined by dotted lines in same panel. Arrowheads in panel d show nuclear accumulation of β-catenin in stroma. Epi: epithelium; ES: endometrial stroma. Bar equals 50 μm.