APCcko uterine stroma is insensitive to estradiol treatment. APCflox/flox uterine size (Panel A, a) and weight (Panel B) is greatly increased after treatment compared to APCcko (Panel A, b & B) mice uteri. Histological sections from untreated control (Panel A, c) and mutant (Panel A, d) uteri were compared with age-matched, estradiol-treated control (Panel A, e) and mutant (Panel A, f) uteri. (Panel C) Western blot analyses of ERα, PR, and Foxo1 in mutant and control uterine lysates (n=3 each). β-actin was used as a loading control. Bar equals 50 μm.