Fig. 5.

BR-C function is required for pupation, elongation, and differentiation of adult legs and wings. Most larvae infected on day 0 of the fifth instar undergo the larval–pupal transition. Compared to a control pupa carrying the TE 3′2J virus (A), a pupa infected with TE 3′2J-Br^as displays short forewings and short malformed legs (B). The arrowhead in B indicates where the wings should extend and meet normally. A mesothoracic leg of a control TE 3′2J infected adult (C) shows the normal number and size of segments, whereas infection with TE 3′2J-Br^as leads to deletions of segments and overall leg malformation (D). Brackets show the normal (C) and shortened (D) tarsi; arrow in D points to an undeveloped mesothoracic leg. (E and F) Although a majority of animals injected as day 2 fourth-instar larvae with control viruses form normal pupae (E), most of those infected with TE 3′2J-Br^as die when trying to ecdyse (F).