Figure-4.

(A) Effect of ENT Alone or with Δ⁴A on the Growth of MDA-MB-231 Xenografts: MDA-MB-231 xenografts were grown in OVX nude mice and treated with vehicle, Δ⁴A (100μg/day), ENT (2.5mg/kg/day) or ENT plus Δ⁴A. The growth rates were not significantly different across the four treatment groups (B) Effect of ENT Alone or with Δ⁴A on the Tumor and Uterine Weights of Mice Bearing MDA-MB-231 Xenografts: Tumor (left y-axis) and uterine (right y-axis) weights were measured at autopsy of above mice in 4A (C) Effect of ENT Alone or with Δ⁴A Supplement on the mRNA Expression of PgR and pS2 in MDA-MB-231 Xenografts: RT-PCR analysis. Lane-1) control, 2) Δ⁴A (100 μg/day), 3) ENT (2.5 mg/kg/day), 4) ENT plus Δ⁴A, 5) Δ⁴A plus letrozole (10μg/day) and 6) ENT plus Δ⁴A plus letrozole. A representative gel image shows ERα and aromatase (CYP-19) and 18s ribosomal RNA (rRNA) as loading control.