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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Biochemistry. 2011 Feb 8;50(8):1403–1411. doi: 10.1021/bi101319v

Figure 4.

Figure 4

pH rate profiles for NH2Y• formation in the reactions of NH2Y730-α2 (blue dots) or NH2Y731-α2 (red dots) with F3Y356-β2 in the presence of CDP/ATP. pH dependence of the rate constants from the 1st (A), 2nd (B) and 3rd (C) kinetic phases determined from the tri-exponential fits (Table 1). In (D), the data from panel (C) are overlaid with the pH rate profiles for [14C]-dCDP formation with F3Y356-β2/wt α2 (black dots) and with intein wt-β2/wt α2 (green squares), determined in a previous study (18).