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. Author manuscript; available in PMC: 2012 Mar 1.

Published in final edited form as: Biochemistry. 2011 Feb 8;50(8):1403–1411. doi: 10.1021/bi101319v

Table 1.

Summary of the kinetic parameters for NH₂Y• formation in the reaction of NH₂Y₇₃₀-α2 or NH₂Y₇₃₁-α2 with F₃Y₃₅₆-β2 and CDP/ATP.

	1^st Phase		2^nd Phase		3^rd Phase
pH	k_obs (s⁻¹) ^a	Amp ^b (% Y₁₂₂•)	k_obs (s⁻¹) ^a	Amp ^b (% Y₁₂₂•)	k_obs (s⁻¹) ^a	Amp ^b (% Y₁₂₂•)
NH₂Y₇₃₀-α2
6.85	30.8 ± 3.7	10 ± 1	2.4 ± 0.3	15 ± 2	0.31 ± 0.04	13 ± 2
7.18	41.7 ± 5.0	7 ± 1	2.7 ± 0.3	16 ± 2	0.42 ± 0.05	15 ± 2
7.55	48.9 ± 5.9	6 ± 1	3.8 ± 0.5	12 ± 1	0.52 ± 0.06	10 ± 1
8.15	21.8 ± 2.6	5 ± 1	2.8 ± 0.3	13 ± 2	0.23 ± 0.03	8 ± 1
8.35	32.9 ± 3.9	3 ± 1	1.0 ± 0.1	10 ± 1	0.20 ± 0.02	14 ± 2
8.65	2.7 ± 0.3	2±1	0.38 ± 0.05	9 ± 1	0.11 ± 0.01	9 ± 2

NH₂Y₇₃₀-α2
6.5	24.7 ± 3.0	11 ± 1	1.7 ± 0.2	20 ± 2	0.21 ± 0.03	13 ± 2
6.9	26.2 ± 3.1	6 ± 1	2.5 ± 0.3	18 ± 2	0.40 ± 0.05	12 ± 1
7.5	28.6 ± 3.4	6 ± 1	3.0 ± 0.4	17 ± 2	0.44 ± 0.05	11 ± 1
8.45	7.8 ± 1.0	6 ± 1	1.2 ± 0.1	14 ± 2	0.12 ± 0.02	9 ± 1
8.6	8.3 ± 1.0	5 ± 1	0.80 ± 0.1	12 ± 1	0.09 ± 0.02	13 ± 2
9.2	−	−	−	−	−	−

^a

Estimated errors based on systematic factors.

^b

The amount of NH₂Y• trapped has been reported as a % of total initial Y₁₂₂•, which in these experiments was 4.8 μM.