Table 1. Generation and genotype of chimeric embryos between WT ES cells and tetraploid embryos from Flt1+/lacZ intercrosses.
| Flt1
|
|||||
|---|---|---|---|---|---|
| Stage | No. transferred | No. recovered | +/+ | +/lacZ | lacZ/lacZ |
| E12.5 | 105 | 16 | 2 | 10 | 4 |
| E14.5 | 186 | 16 | 6 | 9 | 1 |
| Total | 291 | 32 | 8 | 19 | 5 |
| 25% | 59% | 16% | |||
Tetraploid chimeric embryos were generated and genotyped as described in Materials and Methods (Fig. 4). The numbers of chimeric embryos transferred into recipient mothers and that of EYFP+ normal embryos recovered at dissection are indicated. No abnormal EYFP+ fetuses were found.