Skip to main content
. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Biochemistry. 2011 Feb 15;50(10):1672–1681. doi: 10.1021/bi101881d

TABLE 3.

Purification of NrdF from E. coli GR536a

Purification step Protein (mg) Total activity (nmol/min) Specific activity (nmol/min/mg) Percent recovery Purification factor
Crude extractb 7100 5400 0.76 100 1
(NH4)2SO4 / G25b 5400 2100 0.39 40 0.5
Ni-NTA 140 1200 8.8 22 12
DEAE 7.1 400 56 7 74
FPLC 1 0.39 230 580 4 780
FPLC 2 0.15 110 720 2 950
a

NrdF was purified from 88 g of cells

b

The results shown for these steps represent the aggregate of 3 separate purifications from ~30 g cells