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. Author manuscript; available in PMC: 2011 Apr 13.
Published in final edited form as: Methods Mol Biol. 2009;555:127–144. doi: 10.1007/978-1-60327-295-7_10

Fig. 10.3. Theophylline-mediated modulation of alternative splicing in vivo.

Fig. 10.3

(A) HeLa cells were transiently transfected with empty vector, pcABT4M or pcABT4Mmu (containing mutations within core theophylline-binding aptamer). Cells were treated with buffer (lanes 4 and 6) or with 1 mM theophylline (lanes 5 and 7). The product of RT-PCR assays of total RNA isolated from each well was analyzed on a 2.5% agarose gel to estimate the effect of theophylline on the efficiency of exon 2 alternative splicing. The PCR-amplified bands corresponding to exon 2-included or -excluded mRNA is indicated. (B) The internal exon 2 exclusion: inclusion ratio for ABT4M or ABT4Mmu in the absence (open box) or presence of theophylline (shaded box) is shown. The data represent mean ± SEM. P < 0.04 versus mutant is significant.