Table 1.
Plasmids and constructs used in this study
| Plasmid/construct | Description | Reference |
|---|---|---|
| pJV53 | Che9c recombination proteins under control of the acetamidase promoter in pLAM12 | [9] |
| pYUB854 | HygR cassette flanked by γδ-res sites and 2 MCSs | [10] |
| pGH542 | Expressing an γδ resolvase and tetracycline resistant | [11] |
| pEN15 | pYUB854 with a 520 bp fragment harbouring groEL1 (+1067/+1587, relative to groEL1) inserted upstream of the HygR cassette and a 555 bp fragment downstream of groEL1 including the STOP codon of groEL1, inserted downstream of the HygR cassette | This paper |
| pMyNT | Mycobacterial overexpression vector | Geerlof et al., unpublished data |
| pMyNT/PrcA-B | Rv2109-2110 in pMYNT, Rv2110 is N-terminally his-tagged | [12] |
| pMyNT/AccD5E5 | Rv3280-3281 in pMYNT. Only his-tagged Rv3280 seems to express using this construct. | This paper |
| pMyNT/AccA3 | Rv3285 in pMyNT | This paper |
| pMyNT/CFP10-ESAT6 | Rv3874-3875 in pMYNT, Rv3874 is N-terminally his-tagged | [12] |
| pMyNT/ACPS | Rv2523 in pMYNT | This paper |