Table 3.
List of test proteins used to validate the groEL1ΔC expression strain
| ORF | Annotation | Description | Expressed ... | Mol. Mass (kDa) | |||
|---|---|---|---|---|---|---|---|
| Rv2109c Rv2110c |
PrcA PrcB |
α- and β-subunit of the mycobacterial proteasome (α7β7β7α7 subunit organisation) | Using native operon content, producing a 730 kDa multimeric complex | 26.8 30.3 |
|||
| Rv3285 Rv3280 |
AccA3 AccD5 |
α- and β-subunit from acyl-CoA carboxylase AccD5-AccA3 complex (α3β3β3α3 subunit organisation) | As monomeric proteins, mixed to form a acyl-CoA carboxylase complex of 740 kDa | 63.8 59.4 |
|||
| Rv3874 Rv3875 |
CFP10 ESAT6 |
Potential virulence factor CFP10-ESAT6 complex | Using native operon content, producing a heterodimeric (1:1) complex | 10.8 9.9 |
|||
| Rv2523c | ACPS | Holo-acyl-carrier protein synthase | As monomeric protein | 14 | |||