Table 5.
Induction of tumor apoptosis by CTL007 is inhibited by anti-CCR2 and CCL2 Abs and excess CCL2a
| Blocking agent | Number of tumor cells/field (10 fields) |
Number of apoptotic cells/field (10 fields) | % of apoptotic cells | % of tumor cell apoptosis inhibition |
|---|---|---|---|---|
| None | 21.8 ± 9.7 | 8.2 ± 9 | 40.5 ± 9.7b | - |
| Control IgG | 21 ± 2.2 | 8.4 ± 1.1 | 40.1 ± 4.9c | - |
| Anti-CCR1 Ab | 22 ± 3.4 | 10.0 ± 1.6 | 45.6 ± 5.0 | -13.7 |
| Anti-CCR2 Ab | 19.6 ± 1.1 | 2.6 ± 0.5 | 13.2 ± 2.3c | 67.1 |
| Anti-CCR3 Ab | 22.4 ± 2.8 | 9.2 ± 1.9 | 40.7 ± 4.2 | 1.5 |
| Anti-CCR5 Ab | 21 ± 2.6 | 9.6 ± 1.8 | 45.5 ± 3.8 | -13.4 |
| Anti-CCR7 Ab | 18.8 ± 2.4 | 7.2 ± 1.6 | 38.0 ± 4.1 | 5.2 |
| Anti-CXCR3 Ab | 21.4 ± 1.5 | 9.2 ± 0.8 | 43.1 ± 1.8 | -7.5 |
| CCL2 | 21.4 ± 4.4 | 3.6 ± 1.1 | 17.2 ± 6.1b | 57.5 |
| Anti-CCL2 Ab | 17.8 ± 2.6 | 2.2 ± 0.4 | 12.7 ± 3.8c | 68.6 |
a Reconstructs consisted of a bottom layer of collagen and fibroblasts, followed by a tumor cell layer and a separating layer of collagen and fibroblasts. Anti-chemokine receptor or control Abs were added, followed by a top layer containing CTL mixed with fibroblasts and collagen (E: T = 3:1). Excess CCL2 (50 ng/ml) was added to the T-cell layer. Percentage of apoptotic tumor cells in 6 day cultures was determined. (b, c)Values with same letter differ significantly from each other (p < 0.05). Data represented are from a single experiment and the Results were confirmed in at least two independent experiments.