Table 5.

Jamestown Canyon virus strains are highly infectious for weanling Swiss Webster mice, are weakly neuroinvasive, and differ in neurovirulence

Virus	Neuroinvasivenessa (log10 PFU)		Neurovirulenceb (log10 PFU)		Phenotypee
	LD50c	ID50d	LD50	ID50	Neuroinvasive	Neurovirulent
JCV/61/CO	4.8	0.8	1.2	0.5	Low	High
JCV/61/CO-cl	> 4	0.9	0.4	0.4	Low	High
JCV/03/CT	> 5	< 0	3.7	0.3	Low	Low
JCV/03/CT-cl	> 5	< 0	> 4	-0.5	Low	Low
JCV/04/CT-cl	> 5	0.3	1.4	< -1	Low	High

^a Groups of weanling Swiss Webster mice (21-23 days old) were inoculated intraperitoneally with 100 μL of diluted virus (0 - 5 log₁₀ PFU).

^b Groups of six weanling Swiss Webster mice (21-23 days old) were inoculated intracerebrally with 10 μL of diluted virus (-1 - 4 log₁₀ PFU).

^c LD₅₀ = Lethal dose 50%.

^d ID₅₀ = Infectious dose 50%. Infection was defined by the development of clinical disease and/or a four-fold increase in neutralizing antibody titers.

^e Neuroinvasive phenotype: Low = LD₅₀ > 3.5 log₁₀ PFU. Neurovirulent phenotype: Low = LD₅₀ > 3.5 log₁₀ PFU; High = LD₅₀ < 1.5 log₁₀ PFU.