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. 2011 Apr 2;11:120. doi: 10.1186/1471-2407-11-120

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Copyright ©2011 Lechler et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of survivin knock down on cell cycle distribution in chondrosarcoma cells. SW1353 were transfected with siRNA targeting survivin and cell cycle distribution was determined by PI staining and FACS analysis after 24 hours. Both attached and detached cells were collected for the FACS analysis. GFP was transfected as control. Original dot blots and measured gates (left) and resulting histograms (right) are shown. The second peak of the resulting histogram represents the G2/M-phase fraction. The original results of one representative experiment are shown.